Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea

Mathieu Cladière (1, 2) , Grégoire Delaporte (1, 2) , Even Le Roux (1, 2) , Valérie Camel (1, 2)

1 GENIAL - Ingénierie, Procédés, Aliments
2 Université Paris-Saclay

This study attempts at uniting the analysis of four different classes of contaminants for both liquid and solid tea samples. A total of 32 compounds, classified as pesticides, mycotoxins, process-induced toxicants or packaging contaminants, were carefully chosen for their diversity of structures and physicochemical properties. The proposed method combines a sample treatment strategy coming from metabolomics with liquid chromatography analysis using a silica bonded C18-pentafluorophenyl column coupled to high resolution mass spectrometry. For tea brew, dilute and shoot method provides good quantification (70–120% recoveries and <20% RSD) for more than 80% of compounds. For tea leaves, strong matrix effects are observed, thus, matrix-matched calibration is required to reach good performances, i.e. 63% of compounds quantified and 81% detected at 10 µg/kg. Finally, method performances were evaluated against existing regulations, and it appears that 69% of contaminants are quantified and 91% detected at levels lower than their respective European regulation limits.

Mots clés: Food safety; Multi-residue analysis; UHPLC-TOF-MS; tea leaves; C18-PFP; microbiological safety; liquid chromatography; acrylic acid amide; food intake

Mots clés: the; sécurité microbiologique des aliments; alimentation; chromatographie liquide; acrylamide

Domaines: Chimie analytique

Mathieu Cladière, Grégoire Delaporte, Even Le Roux, Valérie Camel. Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea. Food Chemistry, 2018, 242, pp.113-121. ⟨10.1016/j.foodchem.2017.08.108⟩. ⟨hal-01637734⟩