Skip to Main content Skip to Navigation
Journal articles

BK/TD models for analyzing in vitro impedance data on cytotoxicity.

Abstract : The ban of animal testing has enhanced the development of new in vitro technologies for cosmetics safety assessment. Impedance metrics is one such technology which enables monitoring of cell viability in real time. However, analyzing real time data requires moving from static to dynamic toxicity assessment. In the present study, we built mechanistic biokinetic/toxicodynamic (BK/TD) models to analyze the time course of cell viability in cytotoxicity assay using impedance. These models account for the fate of the tested compounds during the assay. BK/TD models were applied to analyze HepaRG cell viability, after single (48h) and repeated (4 weeks) exposures to three hepatotoxic compounds (coumarin, isoeugenol and benzophenone-2). The BK/TD models properly fit the data used for their calibration that was obtained for single or repeated exposure. Only for one out of the three compounds, the models calibrated with a single exposure were able to predict repeated exposure data. We therefore recommend the use of long-term exposure in vitro data in order to adequately account for chronic hepatotoxic effects. The models we propose here are capable of being coupled with human biokinetic models in order to relate dose exposure and human hepatotoxicity.
Document type :
Journal articles
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download

https://hal-agroparistech.archives-ouvertes.fr/hal-01146616
Contributor : Alexandre Pery <>
Submitted on : Thursday, May 28, 2020 - 10:46:00 AM
Last modification on : Monday, August 31, 2020 - 5:19:28 PM

File

2015_Teng_Toxicology Letters_1...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Citation

S Teng, S Barcellini-Couget, R Beaudouin, C Brochot, G Desousa, et al.. BK/TD models for analyzing in vitro impedance data on cytotoxicity.. Toxicology Letters, Elsevier, 2015, 235 (2), pp.96-106. ⟨10.1016/j.toxlet.2015.03.011⟩. ⟨hal-01146616⟩

Share

Metrics

Record views

308

Files downloads

36