Intensifying Effect of Instant Controlled Pressure Drop (DIC) Pre-Treatment on Hesperidin Recovery from Orange Byproducts: In Vitro Antioxidant and Antidiabetic Activities of the Extracts

Mariem Ben Abdallah , Morad Chadni , Nouha M’hiri , Fanny Brunissen , Nesrine Rokbeni , Karim Allaf , Colette Besombes (1) , Irina Ioannou (2) , Nourhene Boudhrioua

1 LaSIE - Laboratoire des Sciences de l'Ingénieur pour l'Environnement - UMR 7356
2 ABI - Agro-Biotechnologies Industrielles

The orange byproduct is a widely accessible and valuable source of functional phenolic compounds, particularly hesperidin. Hesperidin extraction remains a challenging phase in its valorization chain due to its low solubility and limited extractability in solvents. This work aims to examine the effect of conventional solvent extraction (CSE) compared to emerging and innovative extraction methods: accelerated solvent extraction (ASE) and ultrasound-assisted extraction (UAE) when applied with or without a pretreatment process of instant controlled pressure drop (DIC) to intensify extraction, antioxidant, and antidiabetic activities. The total phenols, flavonoids, hesperidin contents, radical scavenging activities, iron chelating activity, and in vitro α-amylase inhibition of the extracts were determined for CSE (80%, 70 °C), UAE (ethanol 80%, 70 °C, 200 W), and ASE (ethanol 60%, 100 °C, 100 bars) with or without DIC pretreatment (pressure = 0.4 MPa, total thermal time = 30 s). The hesperidin amounts obtained were 0.771 ± 0.008 g/100 g DM, 0.823 ± 0.054 g/100 g DM, and 1.368 ± 0.058 g/100 g DM, for CSE, UAE, and ASE, respectively. DIC pretreatment of orange byproducts increased hesperidin recovery by 67%, 25.6%, and 141% for DIC-CSE, DIC-UAE, and DIC-ASE, respectively. The DPPH and ABTS radical scavenging and iron chelating activities of extracts were also significantly enhanced, and the in vitro antidiabetic activity of extracts was preserved.

Mots clés: orange byproducts; hesperidin; antioxidant activity; antidiabetic activity; conventional solvent extraction; accelerated solvent extraction; ultrasound-assisted extraction; instant controlled pressure drop

Domaines: Sciences de l'ingénieur [physics]; Génie des procédés

Mariem Ben Abdallah, Morad Chadni, Nouha M’hiri, Fanny Brunissen, Nesrine Rokbeni, et al.. Intensifying Effect of Instant Controlled Pressure Drop (DIC) Pre-Treatment on Hesperidin Recovery from Orange Byproducts: In Vitro Antioxidant and Antidiabetic Activities of the Extracts. Molecules, 2023, 28 (4), pp.1858. ⟨10.3390/molecules28041858⟩. ⟨hal-03992526⟩