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Journal articles

Protein composition of oil bodies from mature Brassica napus seeds

Abstract : Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped proteins. An exhaustive description of the protein composition of rapeseed OBs from two double-zero varieties was achieved by a combination of proteomic and genomic tools. Genomic analysis led to the identification of sequences coding for major seed oil body proteins, including 19 oleosins, 5 steroleosins and 9 caleosins. Most of these proteins were also identified through proteomic analysis and displayed a high level of sequence conservation with their Arabidopsis thaliana counterparts. Two rapeseed oleosin orthologs appeared acetylated on their N-terminal alanine residue and both caleosins and steroleosins displayed a low level of phosphorylation.
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https://hal-agroparistech.archives-ouvertes.fr/hal-03659048
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Submitted on : Wednesday, May 4, 2022 - 2:44:59 PM
Last modification on : Saturday, May 7, 2022 - 3:31:36 AM

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Distributed under a Creative Commons Attribution 4.0 International License

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Pascale Jolivet, Céline Boulard, Annick Bellamy, Colette Larré, Marion Barre, et al.. Protein composition of oil bodies from mature Brassica napus seeds. Proteomics, Wiley-VCH Verlag, 2009, 9 (12), pp.3268-3284. ⟨10.1002/pmic.200800449⟩. ⟨hal-03659048⟩

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