HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Oil body proteins sequentially accumulate throughout seed development in Brassica napus

Abstract : Despite the importance of seed oil bodies (OBs) as enclosed compartments for oil storage, little is known about lipid and protein accumulation in OBs during seed formation. OBs from rapeseed (Brassica napus) consist of a triacylglycerol (TAG) core surrounded by a phospholipid monolayer embedded with integral proteins which confer high stability to OBs in the mature dry seed. In the present study, we investigated lipid and protein accumulation patterns throughout seed development (from 5 to 65 days after pollination [DAP]) both in the whole seed and in purified OBs. Deposition of the major proteins (oleosins, caleosins and steroleosins) into OBs was assessed through (i) gene expression pattern, (ii) proteomics analysis, and (iii) protein immunodetection. For the first time, a sequential deposition of integral OB proteins was established. Accumulation of oleosins and caleosins was observed starting from early stages of seed development (12-17 DAP), while steroleosins accumulated later (similar to 25 DAP) onwards. (C) 2011 Elsevier GmbH. All rights reserved.
Document type :
Journal articles
Complete list of metadata

https://hal-agroparistech.archives-ouvertes.fr/hal-03659042
Contributor : Sabine d'Andrea Connect in order to contact the contributor
Submitted on : Wednesday, May 4, 2022 - 2:39:04 PM
Last modification on : Saturday, May 7, 2022 - 3:31:36 AM

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Citation

Pascale Jolivet, Céline Boulard, Annick Bellamy, Benoît Valot, Sabine D’andrea, et al.. Oil body proteins sequentially accumulate throughout seed development in Brassica napus. Journal of Plant Physiology, Elsevier, 2011, 168 (17), pp.2015-2020. ⟨10.1016/j.jplph.2011.06.007⟩. ⟨hal-03659042⟩

Share

Metrics

Record views

0