Skip to Main content Skip to Navigation
Journal articles

In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

Abstract : This work aims to intensify trans-p-coumaric acid (p-CA) heterologous production. p-CA exhibits antimicrobial properties, low hydrosolubility, and retro-inhibition activity, making its heterologous production limited due to its accumulation in the broth. To overcome these limitations, an in-stream product recovery process (ISPR) is proposed and consists in a liquidliquid extraction assisted by a hollow fiber membrane contactor. pH, medium composition, and solvent impacts on extraction performances, were investigated prior to the implementation. The coupling of the fermentation and the membrane-assisted extraction was then studied. Although cells were impaired by shear stress with only 15% of viable cells at the end in the extractive fermentation, the final p-CA concentration was approximatively 89% of the control one, suggesting an intensification of p-CA heterologous production if one factors the proportion of viable cells.
Document type :
Journal articles
Complete list of metadata

https://hal-agroparistech.archives-ouvertes.fr/hal-03651986
Contributor : Irina IOANNOU Connect in order to contact the contributor
Submitted on : Tuesday, April 26, 2022 - 11:27:35 AM
Last modification on : Friday, August 5, 2022 - 2:41:13 PM
Long-term archiving on: : Wednesday, July 27, 2022 - 6:42:48 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2022-10-26

Please log in to resquest access to the document

Identifiers

Citation

Jeanne Combes, Nabila Imatoukene, Marwen Moussa, Nicolas Coquart, Florian Chemarin, et al.. In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor. Separation and Purification Technology, Elsevier, 2022, 293, pp.121083. ⟨10.1016/j.seppur.2022.121083⟩. ⟨hal-03651986⟩

Share

Metrics

Record views

46