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Journal articles
In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor
Abstract : This work aims to intensify trans-p-coumaric acid (p-CA) heterologous production. p-CA exhibits antimicrobial properties, low hydrosolubility, and retro-inhibition activity, making its heterologous production limited due to its accumulation in the broth. To overcome these limitations, an in-stream product recovery process (ISPR) is proposed and consists in a liquidliquid extraction assisted by a hollow fiber membrane contactor. pH, medium composition, and solvent impacts on extraction performances, were investigated prior to the implementation. The coupling of the fermentation and the membrane-assisted extraction was then studied. Although cells were impaired by shear stress with only 15% of viable cells at the end in the extractive fermentation, the final p-CA concentration was approximatively 89% of the control one, suggesting an intensification of p-CA heterologous production if one factors the proportion of viable cells.
https://hal-agroparistech.archives-ouvertes.fr/hal-03651986
Contributor : Irina Ioannou Connect in order to contact the contributor
Submitted on : Tuesday, April 26, 2022 - 11:27:35 AM
Last modification on : Monday, May 9, 2022 - 3:47:58 PM
Contributor : Irina Ioannou Connect in order to contact the contributor
Submitted on : Tuesday, April 26, 2022 - 11:27:35 AM
Last modification on : Monday, May 9, 2022 - 3:47:58 PM