In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor - Archive ouverte HAL Access content directly
Journal Articles Separation and Purification Technology Year : 2022

In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

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Abstract

This work aims to intensify trans-p-coumaric acid (p-CA) heterologous production. p-CA exhibits antimicrobial properties, low hydrosolubility, and retro-inhibition activity, making its heterologous production limited due to its accumulation in the broth. To overcome these limitations, an in-stream product recovery process (ISPR) is proposed and consists in a liquidliquid extraction assisted by a hollow fiber membrane contactor. pH, medium composition, and solvent impacts on extraction performances, were investigated prior to the implementation. The coupling of the fermentation and the membrane-assisted extraction was then studied. Although cells were impaired by shear stress with only 15% of viable cells at the end in the extractive fermentation, the final p-CA concentration was approximatively 89% of the control one, suggesting an intensification of p-CA heterologous production if one factors the proportion of viable cells.
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Dates and versions

hal-03651986 , version 1 (26-04-2022)

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Jeanne Combes, Nabila Imatoukene, Marwen Moussa, Nicolas Coquart, Florian Chemarin, et al.. In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor. Separation and Purification Technology, 2022, 293, pp.121083. ⟨10.1016/j.seppur.2022.121083⟩. ⟨hal-03651986⟩
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