Simultaneous extraction and enzymatic hydrolysis of mustard bran for the recovery of sinapic acid

Valentin Reungoat (1, 2) , Louis M.M. Mouterde (1) , Morad Chadni (1) , Julien Couvreur (1) , Emilie Isidore (1) , Florent Allais (1) , Hélène Ducatel (2) , Irina Ioannou (1)

1 ABI - Agro-Biotechnologies Industrielles
2 EXTRACTIS - Centre de Valorisation des Glucides et des Produits Naturels

The simultaneous extraction and enzymatic hydrolysis of sinapine from a mustard residue was designed for the recovery of sinapic acid, a high value-added phenolic acid. An initial screening allowed the identification of sinapoyl esterase activities in commercial enzymatic cocktails (Depol 740 L, Ultraflo XL, Deltazym VR AC-100, Pectinase-PL "Amano") and in a mono-enzymatic solution of rumen feruloyl esterase. These enzymatic cocktails were not very tolerant to ethanol with a diminution of 70-90% of the activity in presence of 10% (v/v) of ethanol. Different extraction processes on mustard bran were designed depending on the solvent compositions (ethanol 70% (v/v), water with or without sinapoyl esterase), pH (4.3, 7 or 12) and temperatures (50, 75 or 100 °C). Their respective efficiencies were discussed. The implementation of Depol 740 L in water allowed to recover 68% of the accessible sinapic acid (25.4 ± 0.1 μmol/g of bran dry matter) in 2h40 min under mild conditions (pH 7, 50 °C). This efficient biocatalytic production of sinapic acid from mustard feedstock using an enzymatic cocktail paves the way for new developments for the design of an industrial process.

Keywords: Mustard bran; enzymatic cocktails; feruloyl esterase; sinapic acid; phenolic acid extraction

Domains: Biochemistry, Molecular Biology

Valentin Reungoat, Louis M.M. Mouterde, Morad Chadni, Julien Couvreur, Emilie Isidore, et al.. Simultaneous extraction and enzymatic hydrolysis of mustard bran for the recovery of sinapic acid. Food and Bioproducts Processing, 2021, 130, pp.68-78. ⟨10.1016/j.fbp.2021.09.003⟩. ⟨hal-03513107⟩