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Effet de la hauteur de prélèvement sur la composition quantitative et qualitative des polyphénols de l’écorce d’Abies alba Mill.

Abstract : The bark of coniferous trees grown for a commercial purpose is a potential precious source of secondary metabolites such as polyphenols. Tannins are among the polyphenols found in the bark. They are used to manufacture adhesives and resins, but also as tanning agents, antibacterial agents, antifungal agents, antitermite agents and antioxidants. Little information is available on the yield and composition of bark extracts depending on the height at which samples are taken from the trunk and on the presence or absence of branches. The present study examines the variability of the secondary metabolites present in Abies alba bark depending on sampling height on the tree, but also on variability at specific heights among several trees. The aim of the study was to determine which bark fraction contained the greatest quantity of extractible compounds in this species. To this end, eight trees were selected, and a maximum of thirteen discs was cut out along each trunk. The samples were taken down the trunk (30 cm from the ground) and then at different heights. The heights were chosen based on industrial criteria (maximum height for lumber production of for industrial use), but also on physiological parameters (e g height at the base of the crown, height of the first green branch). The samples were ground, and then polyphenols were extracted with water/ethanol (1:1, v/v) in fast extraction conditions at high temperature and under high pressure A first – quantitative – study was carried out to determine the total quantities of extractables present in the bark. The second study was qualitative, and aimed at determining which types of extractables were present in the barks. The extracts were analysed by liquid chromatography coupled to a UV-visible spectrometer and a mass spectrometer. The results showed that the quantity of compounds in the total bark extracts increased proportionally to sampling height. The highest proportion of polyphenols was found in the section below the crown
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Submitted on : Tuesday, June 29, 2021 - 8:44:21 AM
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Pauline Gérardin, Clément Fritsch, Sylvain Cosgun, Maree Brennan, Stéphane Dumarcay, et al.. Effet de la hauteur de prélèvement sur la composition quantitative et qualitative des polyphénols de l’écorce d’Abies alba Mill.. Revue forestière française, AgroParisTech, 2020, 72 (5), pp.411-423. ⟨hal-03273202⟩

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