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Organoid Sample Preparation and Extraction for LC-MS Peptidomics

Abstract : This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based approach allows more peptides to be monitored. Our group has previously used LC-MS for tissue peptidomics and secretion of enteroendocrine peptides from primary culture. Now, we extend the use to organoid models.
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https://hal-agroparistech.archives-ouvertes.fr/hal-03165408
Contributor : Pierre Larraufie Connect in order to contact the contributor
Submitted on : Wednesday, March 10, 2021 - 4:13:07 PM
Last modification on : Thursday, April 22, 2021 - 4:50:15 PM
Long-term archiving on: : Friday, June 11, 2021 - 7:08:38 PM

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Emily Miedzybrodzka, Rachel Foreman, Sam Galvin, Pierre Larraufie, Amy George, et al.. Organoid Sample Preparation and Extraction for LC-MS Peptidomics. STAR Protocols, Elsevier, 2020, 1, ⟨10.1016/j.xpro.2020.100164⟩. ⟨hal-03165408⟩

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