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Journal Articles STAR Protocols Year : 2020

Organoid Sample Preparation and Extraction for LC-MS Peptidomics

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Abstract

This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based approach allows more peptides to be monitored. Our group has previously used LC-MS for tissue peptidomics and secretion of enteroendocrine peptides from primary culture. Now, we extend the use to organoid models.
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hal-03165408 , version 1 (10-03-2021)

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Emily L Miedzybrodzka, Rachel E Foreman, Sam G Galvin, Pierre Larraufie, Amy L George, et al.. Organoid Sample Preparation and Extraction for LC-MS Peptidomics. STAR Protocols, 2020, 1, ⟨10.1016/j.xpro.2020.100164⟩. ⟨hal-03165408⟩

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