Identification of the Structure and Origin of Thioacidolysis Marker Compounds for Cinnamyl Alcohol Dehydrogenase Deficiency in Angiosperms

Hoon Kim (1) , John Ralph (1) , Fachuang Lu (2) , Gilles Pilate (3) , Jean-Charles Leplé (3) , Brigitte Pollet (4) , Catherine Lapierre (4)

1 University of Wisconsin-Madison
2 SCUT - South China University of Technology [Guangzhou]
3 AGPF - Unité de recherche Amélioration, Génétique et Physiologie Forestières
4 INRA, Institut National Agronomique Paris-Grignon, Laboratoire de Chimie Biologique

Molecular marker compounds, derived from lignin by the thioacidolysis degradative method, for cinnamyl alcohol dehydrogenase (CAD) deficiency in angiosperms have been structurally identified as indene derivatives. They are shown to derive from hydroxycinnamyl aldehydes that have undergone 8-O-4-cross-coupling during lignification. As such, they are valuable markers for ascertaining plant responses to various levels of CAD down-regulation. Their derivation illustrates that hydroxycinnamyl aldehydes incorporate into angiosperm lignins by endwise coupling reactions in much the same way as normal monolignols do, suggesting that the hydroxycinnamyl aldehydes should be considered authentic lignin precursors.

Domains: Life Sciences [q-bio]

Hoon Kim, John Ralph, Fachuang Lu, Gilles Pilate, Jean-Charles Leplé, et al.. Identification of the Structure and Origin of Thioacidolysis Marker Compounds for Cinnamyl Alcohol Dehydrogenase Deficiency in Angiosperms. Journal of Biological Chemistry, 2002, 277 (49), pp.47412-47419. ⟨10.1074/jbc.M208860200⟩. ⟨hal-03083482⟩