Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies

Alexandre de Saint Germain (1) , Guillaume Clavé (2) , François-Didier Boyer (2)

1 IJPB - Institut Jean-Pierre Bourgin
2 ICSN - Institut de Chimie des Substances Naturelles

In this chapter, we will describe a method we set up to synthesize two profluorescent strigolactone (SL) mimic probes (GC240 and GC242) and the optimized protocols developed to study the enzymatic properties of various strigolactone receptors. The Arabidopsis AtD14 SL receptor is used here as a model for this purpose.

Mots clés: Strigolactones; Bioactive profluorescent probes; Fluorescence; α/β Hydrolase; Receptor; Enzymatic properties

Domaines: Chimie organique; Sciences du Vivant [q-bio]

Alexandre de Saint Germain, Guillaume Clavé, François-Didier Boyer. Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies. "Strigolactones" edited by SpringerNature, , pp.219-231, 2021, 978-1-0716-1429-7. ⟨10.1007/978-1-0716-1429-7_17⟩. ⟨hal-03011263⟩