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Rate of [15N]leucine incorporation and determination of nitrogenous fractions from gastro-jejunal secretion in fasting humans

Abstract : The aim of this study was to quantify the nitrogen fraction flow rates in gastro-jejunal secretions in fasting humans and to determine the [ 15 N] leucine incorporation into the secreted proteins. A double lumen intestinal perfusion method was used in 5 healthy volunteers. Plasma and gastro-jeju-nal juices were collected during a 15-h intravenous [!SN] leucine infusion. Total, soluble and insoluble nitrogen, amino acids and [ 15 N] leucine enrichment were measured. The total nitrogen flow rate was 7.2 ± 1.9 mmol.h-1 and 58% was ethanol soluble. The amino-acid composition remained constant and glycine was the most abundant. The plasma [!5N] leucine enrichment at the isotopic plateau was 4.8 ± 0.9 mol% excess. The [ 15 N] leucine enrichment in the intestinal chyme increased asymptoti-cally to reach a plateau after 5 h. The [ 15 N] leucine enrichment at the plateau and the fractional synthesis rate of secreted proteins were 1.6 ± 0.5 mol% excess and 21.5 ± 3.3%.h-!, respectively. These results show that the composition of the basal gastro-jejunal secretion is very stable. A part of this secretion is composed of proteins with rapid synthesis rates.
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C. Gaudichon, C. Laurent, S Mahé, L Marks, D Tomé, et al.. Rate of [15N]leucine incorporation and determination of nitrogenous fractions from gastro-jejunal secretion in fasting humans. Reproduction Nutrition Development, EDP Sciences, 1994, 34 (4), pp.349-359. ⟨10.1051/rnd:19940407⟩. ⟨hal-02484919⟩

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