Influences of environmental factors on Lactobacillus reuteri growth and 3‐hydroxypropionic acid production in the context of coupling with extraction

Thi-Lan-Phuong Nguyen (1) , Claire Saulou-Berion (1) , Catherine Béal (1)

1 GMPA - Génie et Microbiologie des Procédés Alimentaires

This work aims at improving glycerol bioconversion into 3-hydoxypropionic acid (3-HP) by Lactobacilllus reuteri. Addition of glucose increases cell proliferation but also decreases the molar ratio 3‐HP/1,3‐PDO. Conversely, lower temperature reduce the growth but gives no impact to bioconversion. Addition of vitamin B12 in the growth phase is not effective to 3‐HP production while the presence of betain plus KCl, 1,2‐PDO and phytone peptone extend the time of glycerol consumption. The selective factors giving positive impacts to the bioconversion will be considered in a second experimental design to optimize the conditions during the bioconversion step for 3‐HP production by L. reuteri.

Keywords: Plackett-Burman experimental design; 3-hydroxypropionic acid; Lactobacillus reuteri

Domains: Life Sciences [q-bio]

Thi-Lan-Phuong Nguyen, Claire Saulou-Berion, Catherine Béal. Influences of environmental factors on Lactobacillus reuteri growth and 3‐hydroxypropionic acid production in the context of coupling with extraction. ECAB 5 The 5 th EUROPEAN CONGRESS OF APPLIED BIOTECHNOLOGY, Sep 2019, Florence, Italy. ⟨hal-02361034⟩