Application of the sale‐down methodology to study the effect of mixing on Trichoderma reesei physiology and enzyme production

Tamiris Roque (1) , Fadhel Ben Chaabane (1) , Frédéric Augier (2) , Catherine Béal (3, 4) , A W Nienow (5)

1 IFPEN - IFP Energies nouvelles
2 IFPEN - IFP Energies nouvelles
3 GMPA - Génie et Microbiologie des Procédés Alimentaires
4 AgroParisTech
5 University of Birmingham [Birmingham]

In this study, the effect of oxygen oscillations on enzyme production has been investigated during cultures of T. reesei. By using 2 bioreactors a decrease of 20% in the specific production rate and yield for long-time cycling exposure to anaerobic conditions (15 min) was observed, whereas a short cycle of anaerobiosis (3-4 min) was sufficient by using 1 bioreactor. Our results indicate the sensitivity of T. reesei to anaerobiosis and the fact that the metabolic response is complex and not immediate because it depends on the distribution of the residence times of the microorganisms in the aerated or non‐aerated zones.

Keywords: Agitation; Fluid dynamic stress; Scale-down; filamentous fungi

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Biotechnology; Engineering Sciences [physics]; Chemical and Process Engineering

Tamiris Roque, Fadhel Ben Chaabane, Frédéric Augier, Catherine Béal, A W Nienow. Application of the sale‐down methodology to study the effect of mixing on Trichoderma reesei physiology and enzyme production. ECAB 5 The 5 th EUROPEAN CONGRESS OF APPLIED BIOTECHNOLOGY, Sep 2019, Florence, Italy. ⟨hal-02361006⟩