Mitigation of hydrodynamics related stress in bioreactors: a key for the scale-up of enzyme production by filamentous fungi

Tamiris Roque (1) , Frédéric Augier (1) , Nicolas Hardy (2) , Fadhel Ben Chaabane (1) , Alvin Nienow (3) , Catherine Béal (2)

1 IFPEN - IFP Energies nouvelles
2 GMPA - Génie et Microbiologie des Procédés Alimentaires
3 University of Birmingham [Birmingham]

Different impellers and stirring conditions are compared at the bench scale in order to identify the relevant hydrodynamic stress parameter that correlates best with the process characteristics, morphology, broth rheology and growth rate as measured during the culture of the filamentous fungi, T. reesei, used for the production of cellulases. A modified Energy Dissipation Circulation Function (EDCF) was the most relevant stress parameter, and its use is validated to predict fairly accurately the same process characteristics in an industrial bioreactor, as well as enzyme production. The results are of great importance for the scale-up of cellulase production for bioethanol manufacture from lignocellulosic biomass.

Keywords: Agitation; Fluid dynamic stress; Scale-up; Filamentous fungi; Enzymes

Domains: Life Sciences [q-bio]; Biotechnology; Engineering Sciences [physics]; Chemical and Process Engineering

Tamiris Roque, Frédéric Augier, Nicolas Hardy, Fadhel Ben Chaabane, Alvin Nienow, et al.. Mitigation of hydrodynamics related stress in bioreactors: a key for the scale-up of enzyme production by filamentous fungi. 16th European Conference on Mixing – Mixing 16, Sep 2018, Toulouse, France. ⟨hal-02360962⟩