Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat - AgroParisTech Accéder directement au contenu
Article Dans Une Revue Nutrition and Metabolism Année : 2017

Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat

Résumé

Background: Toll-like receptor 4 (TLR4), an innate immune receptor, is suspected to play a key role in the postprandial inflammation that is induced by a high-fat meal rich in saturated fatty acids (SFA). Our objective was to test this hypothesis by using a specific competitive inhibitor of TLR4 (INH) vs vehicle (VEH) administered immediately before a high-SFA meal in rats. Methods: First, in a cross-over kinetic study of 12 rats receiving INH and VEH i.v. 10 min before the test meal, we measured plasma inflammatory and vascular markers for 6 h. Then, in 20 rats, 3 h after INH or VEH followed by the test meal (parallel study), we measured the mRNA level of a set of cytokines (Il1-β, Il-6, Tnfα, Mcp-1, Pai-1), and of Tlr4 and Tlr2 in the adipose tissue and the liver, and that of adhesion molecules (Icam-1 and Vcam-1) in the aorta. Results: Plasma IL-6 and PAI-1 increased >4-fold at 3–4 h after test-meals, very similarly after INH as compared to VEH. The expression of TLR2 and of all measured cytokine genes in the adipose tissue was dramatically higher after INH (vs VEH). In the liver, gene expression of Il1-β, Tnfα, Mcp-1 and Tlr2, was also higher after INH, though more moderately, whereas that of Il-6 and Pai-1 was similar between groups. INH did not affect mRNA level of Icam-1 and Vcam-1 in the aorta. Conclusion: TLR4 activation is not specifically required to mediate systemic postprandial inflammation and we propose that TLR2 and TLR4 exert a dual and interdependent mediation of the postprandial inflammatory response, at least in the adipose tissue.
Fichier principal
Vignette du fichier
Hermier NutrMetab 2016 (Postprandial low-grade inflammation - TLR4).pdf (835.79 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01620748 , version 1 (20-10-2017)

Licence

Paternité

Identifiants

Citer

Dominique Hermier, Véronique Mathé, Annaig Lan, Clélia Santini, Jean-François Huneau, et al.. Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat. Nutrition and Metabolism, 2017, 14 (1), pp.1-7. ⟨10.1186/s12986-017-0220-4⟩. ⟨hal-01620748⟩
300 Consultations
147 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More