Insulin effects on sterol regulatory-element-binding protein-1c (SREBP-1c) transcriptional activity in rat hepatocytes.

Dalila Azzout-Marniche 1 D Bécard C Guichard M Foretz 2 P. Ferré 3 F. Foufelle 3
1 PNCA - Physiologie de la Nutrition et du Comportement Alimentaire
2 EMD - [Institut Cochin] Département Endocrinologie, métabolisme, diabète (EMD)
3 CRC - Centre de Recherche des Cordeliers
Abstract : The transcription factor sterol regulatory-element-binding protein-1c (SREBP-1c) plays a major role in the effect of insulin on the transcription of hepatic genes such as glucokinase and fatty acid synthase. We show here in cultured rat hepatocytes that insulin, through activation of the phosphatidylinositol 3-kinase pathway increases the abundance of the precursor form of SREBP-1c in endoplasmic reticulum. This precursor form is then rapidly cleaved, possibly irrespective of the continuous presence of insulin, leading to an increased content of the nuclear mature form of SREBP-1c. Nevertheless, the increased amount of the mature form of SREBP-1c in the nucleus is not a prerequisite for the rapid effect of insulin on the transcription of genes such as glucokinase, suggesting that additional actions of the hormone are involved, such as the activation of the nuclear form of SREBP-1c or of an unidentified SREBP-1c partner.
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Journal articles
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https://hal-agroparistech.archives-ouvertes.fr/hal-01611459
Contributor : Dalila Azzout-Marniche <>
Submitted on : Thursday, October 5, 2017 - 8:30:21 PM
Last modification on : Tuesday, May 28, 2019 - 1:46:49 AM

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  • HAL Id : hal-01611459, version 1
  • PUBMED : 10947952

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AGROPARISTECH | INRA | EPHE | USPC | PSL | CORDELIERS | UPMC | UNIV-PARIS5 | SORBONNE-UNIVERSITE | UNIV-PARIS7 | SU-SCIENCES

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Dalila Azzout-Marniche, D Bécard, C Guichard, M Foretz, P. Ferré, et al.. Insulin effects on sterol regulatory-element-binding protein-1c (SREBP-1c) transcriptional activity in rat hepatocytes.. Biochemical Journal, Portland Press, 2000, pp.389-93. ⟨hal-01611459⟩

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