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Effect of inoculum to substrate ratio (I/S) on municipal solid waste anaerobic degradation kinetics and potential

Abstract : The goal of this study is to evaluate the impact of the inoculum to substrate ratio (I/S) on the anaerobic degradation potential of municipal solid waste (MSW). Reconstituted MSW samples were thus incubated under batch anaerobic conditions and inoculated with an increasing amount of inoculum originating from a mesophilic sludge digester. I/S tested values were 0 (no inoculum added), 0.015, 0.03, 0.06, 0.12, 0.25, 1, 2 and 4 (gVM(inoculum)/gVM(waste)). The results indicate that the apparent maximal rate of dissolved organic carbon accumulation is reached at I/S = 0.12. Under this level, the hydrolysis process is limited by the concentration of biomass and can thus be described as first order kinetics phenomena with respect to biomass for I/S ratios below 0.12. The maximum methane production rate and the minimal latency are reached at a ratio of 2. In addition to that, both methane signature and ARISA show a shift in the methanogenic populations from hydrogenotrophic to acetoclastic.
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Submitted on : Thursday, September 7, 2017 - 6:31:52 PM
Last modification on : Thursday, July 22, 2021 - 10:03:06 AM

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Anthony Boulanger, Elisabeth Pinet, Marielle Bouix, Théodore Bouchez, Alicia A. Mansour. Effect of inoculum to substrate ratio (I/S) on municipal solid waste anaerobic degradation kinetics and potential. Waste Management, Elsevier, 2012, 32 (12), pp.2258 - 2265. ⟨10.1016/j.wasman.2012.07.024⟩. ⟨hal-01583777⟩

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