Identification and application of keto acids transporters in Yarrowia lipolytica

Abstract : Production of organic acids by microorganisms is of great importance for obtaining building-block chemicals from sustainable biomass. Extracellular accumulation of organic acids involved a series of transporters, which play important roles in the accumulation of specific organic acid while lack of systematic demonstration in eukaryotic microorganisms. To circumvent accumulation of by-product, efforts have being orchestrated to carboxylate transport mechanism for potential clue in Yarrowia lipolytica WSH-Z06. Six endogenous putative transporter genes, YALI0B19470g, YALI0C15488g, YALI0C21406g, YALI0D24607g, YALI0D20108g and YALI0E32901g, were identified. Transport characteristics and substrate specificities were further investigated using a carboxylate-transport-deficient Saccharomyces cerevisiae strain. These transporters were expressed in Y. lipolytica WSH-Z06 to assess their roles in regulating extracellular keto acids accumulation. In a Y. lipolytica T1 line over expressing YALI0B19470g, α-ketoglutarate accumulated to 46.7 g\textperiodcenteredL(-1), whereas the concentration of pyruvate decreased to 12.3 g\textperiodcenteredL(-1). Systematic identification of these keto acids transporters would provide clues to further improve the accumulation of specific organic acids with higher efficiency in eukaryotic microorganisms.
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Scientific Reports, Nature Publishing Group, 2015, 5, pp.8138. 〈10.1038/srep08138〉
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https://hal-agroparistech.archives-ouvertes.fr/hal-01564919
Contributeur : Armelle Sielinou <>
Soumis le : mercredi 19 juillet 2017 - 11:34:26
Dernière modification le : mercredi 29 novembre 2017 - 15:40:29

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Hongwei Guo, Peiran Liu, Catherine Madzak, Guocheng Du, Jingwen Zhou, et al.. Identification and application of keto acids transporters in Yarrowia lipolytica. Scientific Reports, Nature Publishing Group, 2015, 5, pp.8138. 〈10.1038/srep08138〉. 〈hal-01564919〉

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