Identification and application of keto acids transporters in Yarrowia lipolytica

Hongwei Guo , Peiran Liu , Catherine Madzak (1) , Guocheng Du , Jingwen Zhou , Jian Chen

1 GMPA - Génie et Microbiologie des Procédés Alimentaires

Production of organic acids by microorganisms is of great importance for obtaining building-block chemicals from sustainable biomass. Extracellular accumulation of organic acids involved a series of transporters, which play important roles in the accumulation of specific organic acid while lack of systematic demonstration in eukaryotic microorganisms. To circumvent accumulation of by-product, efforts have being orchestrated to carboxylate transport mechanism for potential clue in Yarrowia lipolytica WSH-Z06. Six endogenous putative transporter genes, YALI0B19470g, YALI0C15488g, YALI0C21406g, YALI0D24607g, YALI0D20108g and YALI0E32901g, were identified. Transport characteristics and substrate specificities were further investigated using a carboxylate-transport-deficient Saccharomyces cerevisiae strain. These transporters were expressed in Y. lipolytica WSH-Z06 to assess their roles in regulating extracellular keto acids accumulation. In a Y. lipolytica T1 line over expressing YALI0B19470g, α-ketoglutarate accumulated to 46.7 g\textperiodcenteredL(-1), whereas the concentration of pyruvate decreased to 12.3 g\textperiodcenteredL(-1). Systematic identification of these keto acids transporters would provide clues to further improve the accumulation of specific organic acids with higher efficiency in eukaryotic microorganisms.

Mots clés: collabinternat; articlescientifique; ecomic

Domaines: Sciences du Vivant [q-bio]

Hongwei Guo, Peiran Liu, Catherine Madzak, Guocheng Du, Jingwen Zhou, et al.. Identification and application of keto acids transporters in Yarrowia lipolytica. Scientific Reports, 2015, 5, pp.8138. ⟨10.1038/srep08138⟩. ⟨hal-01564919⟩