LACCASE5 Is Required for Lignification of the Brachypodium distachyon Culm

Abstract : The oxidation of monolignols is a required step for lignin polymerization and deposition in cell walls. In dicots, both peroxidases and laccases are known to participate in this process. Here, we provide evidence that laccases are also involved in the lignification of Brachypodium distachyon, a model plant for temperate grasses. Transcript quantification data as well as in situ and immunolocalization experiments demonstrated that at least two laccases (LACCASE5 and LACCASE6) are present in lignifying tissues. A mutant with a misspliced LACCASE5 messenger RNA was identified in a targeting-induced local lesion in genome mutant collection. This mutant shows 10% decreased Klason lignin content and modification of the syringyl-to-guaiacyl units ratio. The amount of ferulic acid units ester linked to the mutant cell walls is increased by 40% when compared with control plants, while the amount of ferulic acid units ether linked to lignins is decreased. In addition, the mutant shows a higher saccharification efficiency. These results provide clear evidence that laccases are required for B. distachyon lignification and are promising targets to alleviate the recalcitrance of grass lignocelluloses.
Type de document :
Article dans une revue
Plant Physiology, American Society of Plant Biologists, 2015, 168, pp.192--U951
Liste complète des métadonnées

https://hal-agroparistech.archives-ouvertes.fr/hal-01563837
Contributeur : Armelle Sielinou <>
Soumis le : mardi 18 juillet 2017 - 10:47:31
Dernière modification le : mardi 10 octobre 2017 - 11:10:44

Identifiants

  • HAL Id : hal-01563837, version 1

Citation

Yin Wang, Oumaya Bouchabke-Coussa, Philippe Lebris, Sébastien Antelme, Camille Soulhat, et al.. LACCASE5 Is Required for Lignification of the Brachypodium distachyon Culm. Plant Physiology, American Society of Plant Biologists, 2015, 168, pp.192--U951. 〈hal-01563837〉

Partager

Métriques

Consultations de la notice

41