LACCASE5 Is Required for Lignification of the Brachypodium distachyon Culm

Yin Wang Oumaya Bouchabke-Coussa Philippe Lebris Sébastien Antelme Camille Soulhat Emilie Gineau Marion Dalmais Abdelafid Bendahmane Halima Morin Gregory Mouille Frédéric Legée Laurent Cezard Catherine Lapierre 1 Richard Sibout
1 IJPB - Institut Jean-Pierre Bourgin
Abstract : The oxidation of monolignols is a required step for lignin polymerization and deposition in cell walls. In dicots, both peroxidases and laccases are known to participate in this process. Here, we provide evidence that laccases are also involved in the lignification of Brachypodium distachyon, a model plant for temperate grasses. Transcript quantification data as well as in situ and immunolocalization experiments demonstrated that at least two laccases (LACCASE5 and LACCASE6) are present in lignifying tissues. A mutant with a misspliced LACCASE5 messenger RNA was identified in a targeting-induced local lesion in genome mutant collection. This mutant shows 10% decreased Klason lignin content and modification of the syringyl-to-guaiacyl units ratio. The amount of ferulic acid units ester linked to the mutant cell walls is increased by 40% when compared with control plants, while the amount of ferulic acid units ether linked to lignins is decreased. In addition, the mutant shows a higher saccharification efficiency. These results provide clear evidence that laccases are required for B. distachyon lignification and are promising targets to alleviate the recalcitrance of grass lignocelluloses.
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Journal articles
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Contributor : Armelle Sielinou <>
Submitted on : Tuesday, July 18, 2017 - 10:47:31 AM
Last modification on : Thursday, February 28, 2019 - 5:45:38 PM

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  • HAL Id : hal-01563837, version 1

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AGROPARISTECH | INRA | UNIV-PARIS-SACLAY

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Yin Wang, Oumaya Bouchabke-Coussa, Philippe Lebris, Sébastien Antelme, Camille Soulhat, et al.. LACCASE5 Is Required for Lignification of the Brachypodium distachyon Culm. Plant Physiology, American Society of Plant Biologists, 2015, 168, pp.192--U951. ⟨hal-01563837⟩

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