Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis - AgroParisTech Accéder directement au contenu
Article Dans Une Revue Frontiers in Microbiology Année : 2017

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Résumé

3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories, focusing mainly on Escherichia coli, Klebsiella pneumoniae, and Saccharomyces cerevisiae. Despite the significant progress made in this field, commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study, we investigated the potential of Bacillus subtilis as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant B. subtilis strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from K. pneumoniae. Genetic engineering, driven by in silico optimization, and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L, in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in B. subtilis. With this relatively high titer in batch, and the robustness of B. subtilis in high density fermentation conditions, we expect that our production strains may constitute a solid basis for commercial production of 3-HP.
Fichier principal
Vignette du fichier
fmicb-08-00638_1.pdf (2.46 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01562111 , version 1 (25-05-2020)

Licence

Paternité - Pas de modifications

Identifiants

Citer

Aida A Kalantari, Tao Chen, Boyang Ji, Ivan A. Stancik, Vaishnavi Ravikumar, et al.. Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis. Frontiers in Microbiology, 2017, 8, pp.638. ⟨10.3389/fmicb.2017.00638⟩. ⟨hal-01562111⟩
127 Consultations
67 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More