[13C] GC-C-IRMS analysis of methylboronic acid derivatives of glucose from liver glycogen after the ingestion of [13C] labeled tracers in rats

Abstract : We developed a complete method to measure low [ 13 C] enrichments in glycogen. Fourteen rats were fed a control diet. Six of them also ingested either [U-13 C] glucose (n = 2) or a mixture of 20 [U-13 C] amino acids (n = 4). Hepatic glycogen was extracted, digested to glucose and purified on anion–cation exchange resins. After the optimization of methylboronic acid derivatization using GC–MS, [ 13 C] enrichment of extracted glucose was measured by GC–C-IRMS. The accuracy was addressed by measuring the enrichment excess of a calibration curve, which observed values were in good agreement with the expected values (R = 0.9979). Corrected delta values were −15.6 ± 1.6 ı 13 C (‰) for control rats (n = 8) and increased to −5 to 8 ı 13 C (‰) ‰ and 12–14 ı 13 C (‰) ‰ after the ingestion of [U-13 C] amino acids or [U-13 C] glucose as oral tracers, respectively. The method enabled the determination of dietary substrate transfer into glycogen. The sequestration of dietary glucose in liver glycogen 4 h after the meal was 35% of the ingested dose whereas the transfer of carbon skeletons from amino acids was only 0.25 to 1%.
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Journal of Chromatography B, Elsevier, 2009, 877 (29), pp.3638-3644. 〈10.1016/j.jchromb.2009.09.005〉
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Catherine Luengo, Dalila Azzout-Marniche, Claire Fromentin, Julien Piedcoq, Sophie Lemosquet, et al.. [13C] GC-C-IRMS analysis of methylboronic acid derivatives of glucose from liver glycogen after the ingestion of [13C] labeled tracers in rats. Journal of Chromatography B, Elsevier, 2009, 877 (29), pp.3638-3644. 〈10.1016/j.jchromb.2009.09.005〉. 〈hal-01547479〉

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