Oleic acid accumulating Camelina produced by CRISPR-Cas9 gene editing
Abstract
The possibility of selectively engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely-related expressed sub-genomes, is an ideal species for the creation of a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta-12-desaturase (FAD2) genes was achieved by CRISPR-Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil (Morineau et al. in press).