Oleic acid accumulating Camelina produced by CRISPR-Cas9 gene editing

Morineau Céline (1) , Bellec Yannick (1) , Frédérique Tellier (1) , Lionel Gissot (1) , Zoltan Z. Kelemen (1) , Fabien Nogué (1) , Jean-Denis Faure (1)

1 IJPB - Institut Jean-Pierre Bourgin

The possibility of selectively engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely-related expressed sub-genomes, is an ideal species for the creation of a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta-12-desaturase (FAD2) genes was achieved by CRISPR-Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil (Morineau et al. in press).

Domains: Botanics

Morineau Céline, Bellec Yannick, Frédérique Tellier, Lionel Gissot, Zoltan Z. Kelemen, et al.. Oleic acid accumulating Camelina produced by CRISPR-Cas9 gene editing . Gordon Conference on Plant Lipids, Jan 2017, Galveston, TX, United States. ⟨hal-01529211⟩