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Dual Fatty Acid Elongase Complex Interactions in Arabidopsis

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Abstract

Very long chain fatty acids (VLCFAs) are required for the synthesis of triacylglycerols, waxes, phospholipids and sphingolipids. Fatty acyl chain length is essential for plant development in particular for membrane trafficking during cell division and cell differentiation [1–3]. VLCFAs are elongated by the sequential addition of two carbons through four successive enzymatic reactions gathered in the endoplasmic reticulum within a protein complex named the elongase. The acyl-CoA dehydratase PASTICCINO2 (PAS2) is involved in the third step of elongation [3]. In order to identify new factors associated with the biosynthesis of VLCFAs, a yeast multicopy suppressor screen with an A. thaliana cDNA library was carried out in phs1 strain. Loss of function of PHS1, the yeast PAS2 ortholog, prevents growth and induces cytokinesis defects. The screen identified the PTPLA as a dehydratase-like gene involved in VLCFA elongation in both yeast and plant. Functional analysis of PTPLA in Arabidopsis demonstrated the existence of a new fatty acid elongase complex activity independent of PAS2-based complex but also uncovered unsuspected regulatory interactions between the two complexes.
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Dates and versions

hal-01528029 , version 1 (26-05-2017)

Identifiers

  • HAL Id : hal-01528029 , version 1

Cite

Céline Morineau, Liojnel Gissot, Yannick Bellec, Frédérique Tellier, Kian Hematy, et al.. Dual Fatty Acid Elongase Complex Interactions in Arabidopsis. 26th International Conference on Arabidopsis Research (ICAR) ,, Jul 2015, Paris, France. ⟨hal-01528029⟩
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