Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp nov., Kazachstania serrabonitensis sp nov and Kazachstania australis sp nov Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov. - Archive ouverte HAL Access content directly
Journal Articles International Journal of Systematic and Evolutionary Microbiology Year : 2016

Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp nov., Kazachstania serrabonitensis sp nov and Kazachstania australis sp nov Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov.

(1) , (1) , (2) , (3) , (3) , (4) , (1) , (3) , (4) , (1)
1
2
3
4

Abstract

Five ascosporogenous yeast strains related to the genus Kazachstania were isolated. Two strains (CLIB 1764(T) and CLIB 1780) were isolated from French sourdoughs; three others (UFMG-CM-Y273(T), UFMG-CM-Y451 and UFMG-CM-Y452) were from rotting wood in Brazil. The sequences of the French and Brazilian strains differed by one and three substitutions, respectively, in the D1/D2 large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS). The D1/D2 LSU rRNA sequence of these strains differed by 0.5 and 0.7 % from Kazachstania exigua, but their ITS sequences diverged by 8.1 and 8.3%, respectively, from that of the closest described species Kazachstania barnettii. Analysis of protein coding sequences of RPB1, RPB2 and EF-1 alpha distinguished the French from the Brazilian strains, with respectively 3.3, 6 and 11.7 % substitutions. Two novel species are described to accommodate these newly isolated strains: Kazachstania saulgeensis sp. nov. (type strain CLIB 1764(T)=CBS 14374(T)) and Kazachstania serrabonitensis sp. nov. (type strain UFMG-CM-Y273(T)=CLIB 1783(T)=CBS 14236(T)). Further analysis of culture collections revealed a strain previously assigned to the K. exigua species, but having 3.8% difference (22 substitutions and 2 indels) in its ITS with respect to K. exigua. Hence, we describe a new taxon, Kazachstania australis sp. nov. (type strain CLIB 162(T)=CBS 2141(T)), to accommodate this strain. Finally, Candida humilis and Candida pseudohumilis are reassigned to the genus Kazachstania as new combinations. On the basis of sequence analysis, we also propose that Candida miller and Kazachstania humilis comb. nov. are conspecific.

Dates and versions

hal-01495114 , version 1 (24-03-2017)

Identifiers

Cite

Noémie Jacques, Véronique Sarilar, Charlotte Urien, Mariana R. Lopes, Camila G. Morais, et al.. Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp nov., Kazachstania serrabonitensis sp nov and Kazachstania australis sp nov Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov.. International Journal of Systematic and Evolutionary Microbiology, 2016, 66 (12), pp.5192--5200. ⟨10.1099/ijsem.0.001495⟩. ⟨hal-01495114⟩
117 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More