Contributions of Edema Factor and Protective Antigen to the Induction of Protective Immunity by Bacillus anthracis Edema Toxin as an Intranasal Adjuvant

Alexandra Duverger 1 Jeanne-Marie Carre 1, 2 Junbae Jee 1 Stephen Leepla 3 Estelle Cormet-Boyaka Wei-Jen Tang 4 Daniel Tomé 2 Prosper Boyaka 1, 5
1 Department of Veterinary Biosciences
2 PNCA - Physiologie de la Nutrition et du Comportement Alimentaire
3 Laboratory Bacterial Diseases
4 Ben May Institute Cancer Research
5 Center for Microbial Interface Biology
Abstract : We have shown that intranasal coapplication of Bacillus anthracis protective Ag (PA) together with a B. anthracis edema factor (EF) mutant having reduced adenylate cyclase activity (i.e., EF-S414N) enhances anti-PA Ab responses, but also acts as a mucosal adjuvant for coadministered unrelated Ags. To elucidate the role of edema toxin (EdTx) components in its adjuvanticity, we examined how a PA mutant lacking the ability to bind EF (PA-U7) or another mutant that allows the cellular uptake of EF, but fails to efficiently mediate its translocation into the cytosol (PA-dFF), would affect EdTx-induced adaptive immunity. Native EdTx promotes costimulatory molecule expression by macrophages and B lymphocytes, and a broad spectrum of cytokine responses by cervical lymph node cells in vitro. These effects were reduced or abrogated when cells were treated with EF plus PA-dFF, or PA-U7 instead of PA. We also intranasally immunized groups of mice with a recombinant fusion protein of Yersinia pestis F1 and LcrV Ags (F1-V) together with EdTx variants consisting of wild-type or mutants PA and EF. Analysis of serum and mucosal Ab responses against F1-V or EdTx components (i.e., PA and EF) revealed no adjuvant activity in mice that received PA-U7 instead of PA. In contrast, coimmunization with PA-dFF enhanced serum Ab responses. Finally, immunization with native PA and an EF mutant lacking adenylate cyclase activity (EF-K346R) failed to enhance Ab responses. In summary, a fully functional PA and a minimum of adenylate cyclase activity are needed for EdTx to act as a mucosal adjuvant.
Keywords : ADP-RIBOSYLTRANSFERASE ACTIVITY HEAT-LABILE TOXIN CHOLERA-TOXIN ESCHERICHIA-COLI FUSION PROTEIN MUCOSAL IMMUNITY DENDRITIC CELLS LETHAL FACTOR TUMOR-CELLS IN-VIVO
Document type :
Journal articles
Complete list of metadatas
https://hal-agroparistech.archives-ouvertes.fr/hal-00967206
Contributor : <>
Submitted on : Friday, March 28, 2014 - 10:35:59 AM
Last modification on : Wednesday, November 29, 2017 - 4:05:27 PM

Links full text

openaccess

Identifiers

Collections

AGROPARISTECH | INRA

Citation

Alexandra Duverger, Jeanne-Marie Carre, Junbae Jee, Stephen Leepla, Estelle Cormet-Boyaka, et al.. Contributions of Edema Factor and Protective Antigen to the Induction of Protective Immunity by Bacillus anthracis Edema Toxin as an Intranasal Adjuvant. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2010, 185 (10), pp.5943-5952. ⟨10.4049/jimmunol.0902795⟩. ⟨hal-00967206⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

338

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD