Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia - CRISTAL-BONSAI Accéder directement au contenu
Chapitre D'ouvrage Année : 2022

Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia

Résumé

B cell receptor (BcR) immunoglobulins (IG) display a tremendous diversity due to complex DNA rearrangements, the V(D)J recombination, further enhanced by the somatic hypermutation process. In chronic lymphocytic leukemia (CLL), the mutational load of the clonal BcR IG expressed by the leukemic cells constitutes an important prognostic and predictive biomarker. Here, we provide a reliable methodology capable of determining the mutational status of IG genes in CLL using high-throughput sequencing, starting from leukemic cell DNA or RNA.
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Dates et versions

hal-03684717 , version 1 (02-06-2022)

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Anne Langlois de Septenville, Myriam Boudjoghra, Clotilde Bravetti, Marine Armand, Mikaël Salson, et al.. Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia. Anton W. Langerak. Immunogenetics, 2453, Springer, pp.153-167, 2022, Methods in Molecular Biology, 978-1-0716-2115-8. ⟨10.1007/978-1-0716-2115-8_10⟩. ⟨hal-03684717⟩
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